Canine sperm viability

The influence of pre- and post-ovulatory insemination on sperm distribution in the oviduct, accessory sperm to the zona pellucida, fertilisation rate and embryo development in sows. For this, ovulation was determined by real-time ultrasound examination combined with progesterone analysis. This upper level value has been extremely valuable for identifying where fertilization will occur. However, I have documented many bitches that have been bred much later in their cycles than the fourth to sixth day after the first rise of progesterone. The manufacturer's directions must be followed carefully for each test. However, when dealing with frozen semen, it is critical to know exactly when ovulation has occurred and deliver the frozen semen to the "eggs in waiting.
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Frozen semen uses liquid nitrogen to take the semen to the extreme temperature of 'F. Physiology and endocrinology of reproduction in female dogs; pp. Progesterone levels are measured using a serum sample. Journal List Acta Vet Scand v. Published online Aug Ask the receptionist to check for your e-mail to receive your discount. Only a few sperm have been detected in the oviductal isthmus, indicating that it is unlikely to be a storage site in the bitch [ 6 , 8 - 10 ].
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Effects of semen extender and semen processing on motility

These collections are more for sentimental reasons, than functionality. For progesterone analysis, a competitive enzyme immunoassay Immulite, Siemens Diagnostics; detection limit 0. His major clinical interests include canine reproduction and ultrasonography. While an older male can still be frozen, a better collection can be expected from a young, mature dog two to six years old. By using a clear plastic collection tube, the delivery of these three fractions is easily monitored. Canine semen can also be frozen, allowing long term storage. Of course, dogs are neither hogs nor horses although I must say, I think I've had dogs over the years that might qualify!
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In a normal dog there may be differences in the quality and quantity of the semen from one ejaculate to another even though everything else remains unchanged. Many times we will receive calls for these types of services at the time the bitch begins her estrus cycle. However, it requires anesthesia and surgery. A bitch that has been reproductively normal in the past is best. After insemination the catheter was left for a further 30 minutes in the vagina in order to mimic the duration of the genital lock during natural mating and by this provide sufficient time for the sperm to pass through the cervix.
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